Genome-directed Minimal Primer Set Selection (@BII)

| Introduction | Primer Design | Examples |

Primer Design

Please choose the following parameters:

Primer Length:
Search Window Size from the 3' End of Sequences:

Enter or paste multiple nucleotide sequences of the ORFs in FASTA format below:

(Note: Only the first 4000 nucleotide sequences will be processed if the number of above entered or pasted nucleotide sequences is more than 4000.)

Or

Upload the nucleotide sequence file in FASTA format below:

(Note: Only the first 4000 nucleotide sequences will be processed if the uploaded file contains more than 4000 nucleotide sequences.)


Other Options:

Biological Constraints:
        GC content percentage: from to
        Forming GC clamp at the 3' end of primers:
        Deleting self complementary candidate primers:


Comments, questions? Email Jiren Wang.